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Diagnostics

VetoRapid: A diagnostic tool designed for mastitis pathogen identification​

VetoRapid

Vetorapid is a diagnostic tool that has been designed to aid in the easy identification of the most common bacteria found in mastitic milk samples. It is not intended to replace a high quality diagnostic laboratory but is a useful first line tool in basic identification of the main pathogens that cause clinical mastitis; E.coli, Staphylococci and Streptococci.

Vetorapid-Rapid test for mastitis pathogen identification

Milk Sampling is Key to Mastitis Control

How to correctly take a sample for VetoRapid testing

Before doing any diagnostics, make sure to take milk samples first!

Learn more about the right steps to follow for successful milk sampling below.

Step 1.

Take a clean milk sample from the affected quarter - See milk sampling procedure below for details

Step 2.

Place the sample in the fridge for storage

Step 3.

Prepare your plate in a clean area and take a sterile swab or a loop to dip into the milk sample

Step 4.

Spread the milk sample on the agar plate, using the zig-zag technique

Step 5.

Label the plate and incubate at 35-37°C with the lid on (lid must be facing downwards)

Step 6.

Do the next sample the same way, with a new plate. Only one sample should be analysed per plate

Step 7.

Expect results within 18-36 hours (some cultures can take up to 48 hours)

Step 8.

Follow the diagnostic table, sector by sector.

How to apply the milk sample to the Vetorapid plate

We recommend the use of a loop (metal loop or disposable loop) to apply the milk sample to the Vetorapid plate as results are easier to analyse with this method.

  • Single colonies are easier to detect and there is better differentiation between possible growth and cloudiness caused by the smeared milk
  • Plate the milk as shown in the picture (A) . Do this for all three sections.

(N.B. Sterile cotton swabs can also be used but can cause cloudiness making it harder to differentiate between this and possible growth; however this method can improve sensitivity).

 

Incubation of Vetorapid

Once the sample has been applied to the Vetorapid plate, label and incubate it at 35-37o C with the lid on facing downwards. Results should show within 18-36 hours. Most colonies are detectable within 24 hours.

Vetorapid

To Find Out More About VetoRapid


Download the brochure to find out how to read the results by plate sector and the summary of all streptococcus and enterococcus agar readings:

Gram negative coliform agar (sector 1)

Staphylococcus agar (sector 2)

Streptococcus and enterococcus agar (sector 3) - agar aesculin positive

Streptococcus and enterococcus agar (sector 3) - agar aesculin negative

We are part of the essential milk industry, so we are part of the solution.

We provide you with the tools you need to give the best advice, take preventive action, and make evidence-based decisions.

Win Win
Partnership

Mastitis Prevention

Evidence-Based
Mastitis Therapy

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